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文献和实验Probing and Stripping of Western Blot
270 ml H2OStripping the antibody from Western Blot(Natalya Yudkovsky and Jeff Ranish)Note: these two methods both work, but the first is less time consuming:Easy Stripping Method:1. Put the blot in the following solution
1) After ECL development, wash membrane once for 10min with PBST. 2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag for 30min at 50oC with occasional mixing. For low-abundance antigens, the strip
Stripping for reprobing western blots
minutes. (2) Discard buffer. (3) 5-10 minutes fresh stripping buffer. (4) Discard buffer. (5) 10 minutes PBS (6) 10 minutes PBS (7) 5 minutes TBST (8) 5 minutes TBST Ready
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