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文献和实验DNA Purification-Modified CTAB Procedure
. Resuspend pellet in 5 ml "Ct wash buffer", and 0.1% b-mercaptoethanol and bring to room temperature. Move to orange cap tubes. add 1/5 volume of 5% sarkosyl, set for 15 minutes at room temperature. add 1/7 volume 5M NaCl; mix gently
DNA Purification-Modified CTAB Procedure
wash buffer", and 0.1% b-mercaptoethanol and bring to room temperature. Move to orange cap tubes. 7.add 1/5 volume of 5% sarkosyl, set for 15 minutes at room temperature. 8.add 1/7 volume 5M NaCl; mix gently by inversion. 9.add 1/10
PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS
concentration to ignite). Because of the inherent dangers of heating flammable solvents: 1.) Always conduct experiments in a chemical fume hood 2.) Ensure that the hood is free from all potential sources of ignition including electrical outlets, power strips
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