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革新技术:微毛细管核心技术 节约样本: 更少样品需求, 96孔板实现高通量
更加环保:无需鞘液, 产生更少废液 节省经费: 更少的维持和清洁费用, 无需专门技术人员
操作方便: 简单的软件操作界面, 更少的培训时间
Flow cytometry is an essential tool for in-depth cell analysis. With the capacity to simultaneously measure multiple parameters on hundreds of individual cells per second, flow cytometry offers greater speed, precision, and detail than most other cell analysis methods available to scientists today.
Guava Flow Cytometry Systems:
At the heart of every Guava System is a unique, microcapillary flow cell that eliminates the need for sheath fluid. This translates into smaller samples, less reagents, and minimal waste, saving you both time and money. Plus, since the flow cell is self-aligning and user-replaceable, you can remove it yourself at any time for cleaning and maintenance—no more expense or downtime for service visits. And, by eliminating complicated fluidics, we’ve created a tiny instrument footprint that fits into the tightest spots, saving valuable laboratory space. Walk-away automation options and user-friendly software simplify your research.
Key Features of the PCA-96 System:
Size – it won’t take up much room in your lab:
∙ Tiny footprint
∙ Integrated laptop
Samples – 96-well microplate and 10 sample tubes:
∙ Flexible throughput
∙ Requires minimal sample
∙ Walk-away automation options
Detection – simple options for common assays:
∙ Green (532 nm) excitation laser for many fluorophores
∙ Two color (red and yellow) fluorescence detectors
∙ Forward scatter
Analysis – do more with less:
∙ System runs most Guava Assays
Maintenance – spend less time cleaning and more time working:
∙ System generates <50 µL of overall waste in a typical 8-hour day
∙ Flow cell is user-replaceable
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文献和实验Purpose Flow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based
Indirect flow cytometry (FACS) protocol
for which you have an appropriate centrifuge e.g. test tubes, eppendorf tubes, and 96 well round bottomed microtiter plates. In general,cells should be spun down hard enough that the supernatant fluid can be removed with little loss of cells, but not so hard that the cells
Intracellular Immunofluorescent Staining for Flow Cytometry
to stain intracellularly. In vitro stimulation of cells is usually required for detection of cytokines by flow cytometry since cytokine levels are typically too low in resting cells. Stimulation of cells with the appropriate reagent will depend on the cell
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