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- 库存：
大量
- 英文名：
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
100plates
齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电：021-59588506
Amplite™Renilla萤光素酶报告基因基因检测试剂盒详细如下
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP. Our Amplite™ Renilla Luciferase Reporter Gene Assay Kit is designed to provide a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify Renilla luciferase activity in cell-based assays. Our formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The "glow-type" signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes .
Components：
Component A: 100X Luciferase Substrate (Light-Sensitive) 1 vial (100 µL)
Component B: Assay Buffer 1 bottle (10 mL)
齐源生物专业代理美国AAT Bioquest其它产品
Amplite™Renilla萤光素酶报告基因基因检测试剂盒详细如下
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP. Our Amplite™ Renilla Luciferase Reporter Gene Assay Kit is designed to provide a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify Renilla luciferase activity in cell-based assays. Our formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The "glow-type" signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes .
Components：
Component A: 100X Luciferase Substrate (Light-Sensitive) 1 vial (100 µL)
Component B: Assay Buffer 1 bottle (10 mL)
齐源生物专业代理美国AAT Bioquest其它产品
| QYS-12503 | D-Luciferin, free acid *CAS#: 2591-17-5* | D-荧光素，游离酸荧光素 | 1g |
| QYS-12505 | D-Luciferin, potassium salt *CAS#: 115144-35-9* | D-荧光素，钾盐 | 25mg |
| QYS-12506 | D-Luciferin, potassium salt *CAS#: 115144-35-9* | D-荧光素，钾盐 | 100mg |
| QYS-12507 | D-Luciferin, potassium salt *CAS#: 115144-35-9* | D-荧光素，钾盐 | 1g |
| QYS-12509 | D-Luciferin, sodium salt *CAS#: 103404-75-7* | D-萤光素，钠盐 | 25mg |
| QYS-12510 | D-Luciferin, sodium salt *CAS#: 103404-75-7* | D-萤光素，钠盐 | 100mg |
| QYS-12511 | D-Luciferin, sodium salt *CAS#: 103404-75-7* | D-萤光素，钠盐 | 1g |
| QYS-12512 | D-Luciferin phosphate *CAS 145613-12-3* | D-荧光素磷酸酯 | 1mg |
| QYS-12513 | D-Luciferin galactoside *CAS 131474-38-9* | D-萤光素半乳糖苷 | 5mg |
| QYS-12514 | D-Luciferin methyl ester *CAS 73918-26-0* | D-荧光素甲酯 | 5mg |
| QYS-12515 | D-Luciferin ethyl ester *CAS 135251-85-3* | D-荧光素乙酯 | 5mg |
| QYS-12516 | D-Luciferin acetate | 乙酸D-荧光素乙酸 | 5mg |
| QYS-12518 | Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™萤光素酶报告基因基因检测试剂盒 | 1plate |
| QYS-12519 | Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™萤光素酶报告基因基因检测试剂盒 | 10plates |
| QYS-12520 | Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™萤光素酶报告基因基因检测试剂盒 | 100plates |
| QYS-12530 | Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™高斯荧光素酶报告基因基因检测试剂盒 | 1plate |
| QYS-12531 | Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™高斯荧光素酶报告基因基因检测试剂盒 | 10Plates |
| QYS-12532 | Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™高斯荧光素酶报告基因基因检测试剂盒 | 100plates |
| QYS-12535 | Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™Renilla萤光素酶报告基因基因检测试剂盒 | 1plate |
| QYS-12536 | Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™Renilla萤光素酶报告基因基因检测试剂盒 | 10plates |
| QYS-12537 | Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* | Amplite™Renilla萤光素酶报告基因基因检测试剂盒 | 100plates |
| QYS-12601 | Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence* | Amplite™荧光β-半乳糖苷酶检测试剂盒 | 500Tests |
| QYS-12602 | Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence* | Amplite™荧光神经氨酸酶测定试剂盒 | 200Tests |
| QYS-12603 | Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence* | Amplite™荧光β-半乳糖苷酶检测试剂盒 | 200Tests |
| QYS-12604 | Amplite™ Colorimetric Beta-Galactosidase Assay Kit | Amplite™比色β-半乳糖苷酶检测试剂盒 | 200Tests |
| QYS-12609 | NTA maleimide | NTA马来酰亚胺 | 5mg |
| QYS-12610 | HIS Lite™ Cy3 Bis NTA-Ni Complex | HIS Lite™Cy3 Bis NTA-Ni复合物 | 1mg |
| QYS-12613 | HIS Lite™ Cy5 Bis NTA-Ni Complex | HIS Lite™Cy5 Bis NTA-Ni复合物 | 1mg |
| QYS-13000 | Benzoyl-Leu-Gly-Arg-Aminoluciferin | 苯甲酰-Leu-甘氨酸-精氨酸-氨基荧光素 | 1mg |
| QYS-13001 | BOC-Leu-Gly-Arg-Aminoluciferin | BOC-Leu-Gly-Arg-氨基荧光素 | 1mg |
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文献和实验相关实验
生物发光是一种依赖于酶和底物的相互作用来产生光的化学过程。所需的酶被称为荧光素酶，底物因荧光素酶的类型而不同（如荧光素）。 常用荧光素酶信息表： 其中最有代表性的是来自萤火虫体内（Firefly）和海肾（Renilla）体内的两类萤光素酶，分别命名为萤火虫荧光素酶（FLuc）和海肾荧光素酶（RLuc），同时近年来研究得较多的是来源于高斯氏菌的高斯荧光素酶（GLuc）。 萤火虫荧光素酶（FLuc）作为最通用和最常见的报告基因，该蛋白质不需要翻译后修饰即可获得酶活性，可用于原核和真核细胞。FLuc
。实验报告基因用于测试实验条件下基因的表达，而对照报告基因作为内对照，以使实验报告基因测试的结果归一化。作归一化可消除实验过程中减弱实验准确度的变化，如培养细胞的数量、细胞转染 及裂解的效率等。在研究转录调控启动子活性中，具体实验步骤如下。 1. 质粒转染细胞 1) 细胞 铺板：将细胞按70%的汇合度接种到96孔板。 2) 转染：16h后转染萤光素酶报告基因 质粒和RNA，每个样品设置6个复孔 a) 配制DNA和转染试剂 : 96孔板转染质粒时
【求助】topflash/foplash的luciferase opening reading fream针对的就是firefly萤光素酶吗？
lope 请教各位大侠： 购买的UPSTATE的TOPFLASH/FOPFLASH质粒，准备做双荧光素酶报告基因检测，看文献上都只是用这个质粒和Renilla的载体共转染细胞，是否这个质粒本身就携带了Firefly萤光素酶呢？我看了说明书，只是说two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse
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Amplite™Renilla萤光素酶报告基因基因检测试剂盒
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