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- 文献和实验
- 技术资料
- 保存条件:
Powder: -20°C, 3 years; 4°C, 2 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
892128-60-8
- 规格:
10 mM * 1 mL/1 mg/2 mg/5 mg/10 mg/25 mg/50 mg
| 规格: | 10 mM * 1 mL | 产品价格: | ¥854.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥588.0 |
| 规格: | 2 mg | 产品价格: | ¥765.0 |
| 规格: | 5 mg | 产品价格: | ¥997.0 |
| 规格: | 10 mg | 产品价格: | ¥1895.0 |
| 规格: | 25 mg | 产品价格: | ¥3790.0 |
| 规格: | 50 mg | 产品价格: | ¥5495.0 |
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LY2334737
CAS No. : 892128-60-8
MCE 国际站:LY2334737
产品活性:LY2334737 是一种核苷类似物,也是一种具有口服活性的 Gemcitabine 的前体。LY2334737 对肠道病毒 A71 (EV-A71) 感染具有抑制活性。LY2334737 具有抗病毒和抗癌作用。
研究领域:Cell Cycle/DNA Damage | Anti-infection
作用靶点:Nucleoside Antimetabolite/Analog | Enterovirus
In Vitro: Five cell lines that express CES2 responded to LY2334737 treatment. LY2334737 is less cytotoxic to a SK-OV-3 CES2 knockdown than parental cells. The drug response of CES2-transfected HCT-116 cells correlated with CES2 expression level. Bystander studies show statistically greater PC-3-GFP growth inhibition by LY2334737 when cells are cocultured with CES2 and not mock transfectants.
In Vivo: Oral treatment of xenograft models with 3.2 mg/kg LY2334737 once a day for 21 days shows greater tumor growth inhibition of CES2 transfectant than the mock transfectant.
Metronomic LY2334737 administration causes increased blood flow in luciferase-tagged LM2-4 tumor xenografts, and this effect, readily measured using contrast micro-ultrasound, coincided with a relative increase in tumor bioluminescence.
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文献和实验ly86400 wrote: 啊?我们就是用常规做法。。 检测的效果还可以啊~你的检测时间是什么概念啊? 就是用WB检测p-AKT的时间,有的paper是0,30,60,120分钟,看这段时间的p-AKT的变化,有的却是检测24小时的p-AKT,我对于这个时间检测不是很懂,请赐教! 1112234 我也正要做这方面的实验呢,想请教一下楼主,最后你的时间是如何确定的呀?
58、CD60、CD65- CD67、CD75- CD78、CD84、CD85、CD89、CD92- CD94、CD96、CD97、CD99- CD101、CD108、CD109、CD136、CD139、CD146、CD148- CD150、CD155、CD163- CD165。(二) 小鼠的Ly系统与CD关系最初应用mAb所鉴定的小鼠免疫细胞表面标记称为淋巴细胞抗原 (lymphocyte antigen,Ly),Ly系列包括Ly1- Ly81(见附表2)。目前大多数Ly编号已有小鼠CD的相应编号(见
膜,转膜时间75min。转膜完成后丽春红染色试剂对膜进行染色,观察转膜效果。同时做好泳道标记,准备预实验时进行按照纵向泳道剪膜。3.6 封闭:将膜完全浸没封闭液(德元国际 Cat# LY7141)中室温轻摇60min。3.7 一抗孵育:用TBST稀释一抗,室温孵育10min,放4℃过夜。3.8 洗膜第二天从4度拿出膜,在室温孵育30min。洗膜:TBST洗膜5次,每次10min。3.9 二抗孵育:用5%脱脂奶粉-TBST稀释二抗,山羊抗兔IgG(H+L) HRP 山羊抗小鼠IgG(H+L
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