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Please store the product under the recommended conditions in the Certificate of Analysis.
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货期：1-2天
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MedChemExpress LLC
- 规格：
25 mg/50 mg/100 mg
| 规格： | 25 mg | 产品价格： | ￥500.0 |
|---|---|---|---|
| 规格： | 50 mg | 产品价格： | ￥750.0 |
| 规格： | 100 mg | 产品价格： | ￥1200.0 |
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Recombinant Proteinase K
MCE 国际站：Recombinant Proteinase K
产品活性：Recombinant Proteinase K 是一种丝氨酸蛋白酶 (serine protease)，它能裂解脂肪族和芳香族氨基酸的羧基端肽键。Recombinant Proteinase K 可用于消化蛋白质和清除核酸制剂中的污染。
研究领域：Metabolic Enzyme/Protease
作用靶点：Ser/Thr Protease
In Vitro: Proteinase K digestion
1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.
2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 µl aliquot to get the final concentrations of 2, 4 and 8 µg/ml. Reactions are carried out at 37°C for 30 minutes.
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文献和实验Proteinase K is a serine protease and the main proteolytic enzyme produced by the fungus Tritirachium album Limber (1 ). The enzyme has abroad specificity, cleaving peptide bonds C-terminal to a number of amino acids. The enzyme is produced
RNA Extraction by the Proteinase K Method
. Next the polysomes are dissociated with SDS and proteinase K and finally the protein is removed by several phenol/choloroform extractions (1 ,2 ).
分子量: 28.9 kDa 单位定义: 在250 μl反应体系, 37℃条件下1分钟内能分解释放相当于1 μmol酪氨酸的folin-阳性氨基酸和肽所需要的蛋白酶K的量定义为一个单位(1) 贮存条件: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2和50%甘油。贮存于-20℃ Proteinase K，中文名为蛋白酶K。不含DNase，不含
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