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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
6个月
- 英文名:
Recombinant Shigella flexneri Protein mxiH (mxiH)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
OthersUniprot ID
P0A223Gene Names
mxiHAlternative Name(s)
mxiH; CP0137; Protein MxiHLead Time
3-7 business daysOrganism
Shigella flexneriSource
E.coliExpression Region
1-83aaProtein Length
Full LengthTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNPQLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFRMW
16.7 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
LiquidBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验删除突变的drs基因分析揭示,C末端区以及3个一致重复的N末端区都出现凋亡。Caspase-12、Caspase –9以及Caspase-3都继续被drs激活,Caspase--3,和Caspase-9的抑制剂都抑制drs引起的凋亡。在凋亡过程中没有看到因drs引起线粒体细胞色素C释放到细胞质去,这表明线粒体途径不是drs介导的凋亡,而且,研究人员发现,Drs蛋白能与定位在内质网的凋亡蛋白ASY/Nogo-B/RTN-x(S)结合,并且这些基因共同表达增加了凋亡的效果。这项研究结果提示,由ASY
。Smac前体在胞质中合成,继而转运到线粒体。成熟型的Smac通过N端的Ala―Val―Pro―Ile(AVPl)序列与细胞内的凋亡抑制蛋白(inhibitorofapoptosis protein,IAP)结合,后者是内源性的caspase抑制剂。1AP与caspase―9或caspase―3结合后将其灭活,从而抑制凋亡的发生。Smac与IAP的结合后,caspase―9和caspase―3释放,然后促发凋亡。 (二)caspase非依赖通路 尽管caspase是凋亡信号起始
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
). 4. In addition to studying the response of BMMs isolated from knock-out mice, theDCC can be used to characterize the chemotactic response of cells expressingtagged proteins introduced either by microinjection of recombinant protein ordeoxyribonucleic acid (16
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