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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Collagen alpha-2 (VI) chain (COL6A2), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥46572.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥11088.0 |
| 规格: | 20μg | 产品价格: | ¥3960.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
COL6A2Uniprot No.:
P12110别名:
CO6A2_HUMAN; COL6A2; Collagen alpha 2(VI) chain; Collagen alpha-2(VI) chain; collagen type VI alpha 2; Collagen VI alpha 2 polypeptide; human mRNA for collagen VI alpha 2 C terminal globular domain; PP3610种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Mammalian cell分子量:
37.3 kDa表达区域:
941-1016aa氨基酸序列:
ELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIR蛋白标签:
C-terminal hFc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Collagen VI acts as a cell-binding protein.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Amino acid sequence of the triple-helical domain of human collagen type VI." Chu M.-L., Conway D., Pan T.-C., Baldwin C., Mann K., Deutzmann R., Timpl R. J. Biol. Chem. 263:18601-18606(1988)Function:
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文献和实验X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白
CD49a/CD49b/CD49c/CD49d/CD49e/CD49f分子
originally identified on activated human T cells, later on fibroblasts, platelets, others. Six forms, VLA1 to VLA6, have been identified, each with distinct alpha chain (numbered alpha-1 to alpha-6) associated with a common beta chain. CD49a - Laminin
, Simultaneous purification and renaturation of recombinant human interferon alpha expressed by E. coli by high-performance hydrophobic interaction chromatography, Chinese J. Chromatogr. 19 (2001) 301-303. 38. E. Kurimoto, T. Harada, A. Akiyama, T. Sakai, K
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