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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Interleukin-13 (IL13), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥27960.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6654.0 |
| 规格: | 20μg | 产品价格: | ¥2376.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
IL13Uniprot No.:
P35225别名:
IL-13种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Mammalian cell分子量:
42 kDa表达区域:
21-132aa氨基酸序列:
TVIALTCLGGFASPGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDL蛋白标签:
C-terminal hFc-Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
ImmunologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Coexpression of the interleukin-13 and interleukin-4 genes correlates with their physical linkage in the cytokine gene cluster on human chromosome 5q23-31." Dolganov G., Bort S., Lovett M., Burr J., Schubert L., Short D., McGurn M., Gibson C., Lewis D.B. Blood 87:3316-3326(1996)Function:
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文献和实验开辟先河!STM 背靠背报道非特异性靶点组合 CAR-T 疗法,抗癌效果更好,也更持久
CAR-T 细胞只针对神经系统中的癌细胞。 图片来源:Science Translational Medicine 通过整合来自多个抗原和多个细胞的信息,这些回路本质上提高了精细识别肿瘤这一复杂组织的能力,从而可以更安全、更精确地识别和攻击肿瘤。实验结果表明,EGFRvIII-synNotch -a-EphA2/IL13Ra2 CAR-T 细胞能够有效和完全清除 GBM6 肿瘤,而不会杀死周围的正常组织或体内其他缺少共定位引物抗原的 EphA2 - 或 IL13Ra2 阳性细胞。 图片来源
://pid.nci.nih.gov 简介:由NCI和Nature 共同创立,提供已知的人体细胞信号转导、调节活动及主要细胞生命过的蛋白质路径网,可通过输入某个分子名或代谢过程名称进行查询。 10.UniHI(Unified Human Interactome database) 网址:http://www.unihi.org 简介:人体蛋白-蛋白相互作用数据库,可根据蛋白质名称、代谢路径等进行查询。 11.VirHostNet
X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白
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