/5a重组蛋白ns12.7;12.7 kDa accessory proteinBovine coronavirus Non-structural protein of 12.7 kDa(5a)蛋白产品图
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/5a重组蛋白ns12.7;12.7 kDa accesso

ry proteinBovine coronavirus Non-structural protein of 12.7 kDa(5a)蛋白
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  • ¥1344
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  • CSB-EP848658BJF
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Bovine coronavirus Non-structural protein of 12.7 kDa (5a)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Bovine coronavirus Non-structural protein of 12.7 kDa (5a)

    品名简称:

    Recombinant Bovine coronavirus Non-structural protein of 12.7 kDa

    货号:

    CSB-EP848658BJF

    规格:

    20μg

    价格:

    1344

    LC-MS/Western Blot:

    N/A

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    5a

    Uniprot No.:

    Q8V434

    别名:

    ns12.7;12.7 kDa accessory protein

    种属:

    Bovine coronavirus (strain 98TXSF-110-LUN) (BCoV-LUN) (BCV)

    蛋白长度:

    Full Length

    来源:

    E.coli

    分子量:

    15.1 kDa

    表达区域:

    1-109aa

    氨基酸序列:

    MDIWKPEIKYLRYTNGFNVSELEDACFKFNYKFPKVGYCRVPSHAWCRNQGSFCATLTLYGKSKHYDKYFGVITGFTAFANTVEEAVNKLVFLAVDFITWRRQELNVHG

    蛋白标签:

    C-terminal 6xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    13-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    /

    功能2:

    /

    文献:

    /

    研究领域:

    Microbiology

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    相关实验
    • 植物质膜蛋白的提取和溶解

      的溶解步骤 [18 ] ( 见第 12 章),并且表达丰度低。本章描述了从模式植物拟南芥的根和叶悬浮细胞中纯化 PM 的方案,得到能用于蛋白质组学分析的可溶性 PM 蛋白。水通道蛋白是含有 6 个跨膜 α-螺旋 [ 19~22] ,26~35 kDa 的疏水性质膜镶嵌蛋白(PIP ) ,被用来作为评估相关的提取及溶解方案的对照蛋白质。2. 材料 2.1 生物材料 ( 1 ) 拟南芥属(Heynh.) 植物(生态型为 Wassilewskija 或 Columbia ) , 培养

    • Western Blot Protocols

      SDS-PAGE gel for protein of interest. Typically 12% acrylamide gels are used for high molecular weight (MW) proteins (>50 kDa), 15% gels for mid range MW proteins (15 - 50 kDa) and 20% gels for low MW proteins (2.Remove stacking gel and sides

    • Analysis of Proteins using Small Format 2D Gel Electrophoresis

      dithiothreitol, 2.3% (w/v) SDS) for 7 minutes before embedding in 1% (w/v) agarose prepared in 0.125M Tris-HCl, pH 6.7, 0.1% (w/v) SDS with bromophenol blue to colour. The second dimension slab gels (70 x 80 mm) are prepared in batches of 12 gels using

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    ¥1344