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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bovine coronavirus Non-structural protein of 12.7 kDa (5a)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bovine coronavirus Non-structural protein of 12.7 kDa (5a)品名简称:
Recombinant Bovine coronavirus Non-structural protein of 12.7 kDa货号:
CSB-EP848658BJF规格:
20μg价格:
1344LC-MS/Western Blot:
N/ASDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
5aUniprot No.:
Q8V434别名:
ns12.7;12.7 kDa accessory protein种属:
Bovine coronavirus (strain 98TXSF-110-LUN) (BCoV-LUN) (BCV)蛋白长度:
Full Length来源:
E.coli分子量:
15.1 kDa表达区域:
1-109aa氨基酸序列:
MDIWKPEIKYLRYTNGFNVSELEDACFKFNYKFPKVGYCRVPSHAWCRNQGSFCATLTLYGKSKHYDKYFGVITGFTAFANTVEEAVNKLVFLAVDFITWRRQELNVHG蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
Microbiology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验的溶解步骤 [18 ] ( 见第 12 章),并且表达丰度低。本章描述了从模式植物拟南芥的根和叶悬浮细胞中纯化 PM 的方案,得到能用于蛋白质组学分析的可溶性 PM 蛋白。水通道蛋白是含有 6 个跨膜 α-螺旋 [ 19~22] ,26~35 kDa 的疏水性质膜镶嵌蛋白(PIP ) ,被用来作为评估相关的提取及溶解方案的对照蛋白质。2. 材料 2.1 生物材料 ( 1 ) 拟南芥属(Heynh.) 植物(生态型为 Wassilewskija 或 Columbia ) , 培养
SDS-PAGE gel for protein of interest. Typically 12% acrylamide gels are used for high molecular weight (MW) proteins (>50 kDa), 15% gels for mid range MW proteins (15 - 50 kDa) and 20% gels for low MW proteins (2.Remove stacking gel and sides
Analysis of Proteins using Small Format 2D Gel Electrophoresis
dithiothreitol, 2.3% (w/v) SDS) for 7 minutes before embedding in 1% (w/v) agarose prepared in 0.125M Tris-HCl, pH 6.7, 0.1% (w/v) SDS with bromophenol blue to colour. The second dimension slab gels (70 x 80 mm) are prepared in batches of 12 gels using
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