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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
) directed against cell surface antigens of interest (biotinylated or labeled with an appropriate fluorochrome), and isotype-matched controls The protocol described here uses one biotinylated mAb in combination with streptavidin-Alexa Fluor 488
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
) Alexa Fluor 647 goat anti–mouse IgM (2 mg/ml; Invitrogen, cat. no. A21238) R‐phycoerythrin goat anti–mouse IgG2a conjugate (1.0 mg/ml; Invitrogen, cat. no. P21139
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