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- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
根据瓶身LOT号查询
- 英文名:
Mineral oil
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
8042-47-5
- 规格:
100ML
属性
质量水平
200
无菌性
non-sterile
形式
light oil
储存条件
protect from light
折射率
n20/D 1.467 (lit.)
密度
0.84 g/mL at 25 °C (lit.)
格式
neat
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
应用
注意
分析说明
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文献和实验Whole-Mount Kidney Clearing and Visualization Reveal Renal Sympathetic Hyperinnervation in Heart Failure Mice.
Developing a three-dimensional (3D) visualization of the kidney at the whole-mount scale is challenging. In the present study, we optimized mouse whole-mount kidney clearing, which improved the transparency ratio to over 90% based on organ-specific perfusion (OSP)-clear, unobstructed brain imaging cocktails and computational analysis (CUBIC). The optimized OSP-CUBIC-compatible 3D immunostaining and imaging simultaneously visualized the high-resolution 3D structure of the whole-mount renal microvascular, glomerulus, and accompanying wrapped traveling sympathetic nerves in mice. A mouse model of pressure overload-induced heart failure (HF) was then established by minimally invasive transverse aortic constriction (MTAC). Further 3D quantification revealed renal sympathetic hyperinnervation (6.80 ± 1.04% vs. 3.73 ± 0.60%, P < 0.05) in mice with HF. In conclusion, this newly developed whole-organ tissue clearing and imaging system provides comprehensive information at the whole-mount scale and has great potential for kidney research. Our data suggest that renal sympathetic hyperinnervation is involved in HF associated with renal dysfunction.
licanming 请问大家用sigma公司的PMA时,储存液的浓度是配多少的?保存期多长?我是准备用DMSO配,我看说明书的意思是储存液浓度为20mM,但我工作液的浓度只要100nM,故很难分装和稀释。如果储存液浓度低一点,会不会对保存时间产生影响啊?我在园子里搜过,有用1mg/ml的,但我不知他是否用sigma公司的。 licanming 自己顶一下 amygdala 对于保存
:Formerly listed as Type III 溶解性:溶于水,参考浓度2mg/ml 储存条件:2-8℃ From Sigma D1626 Salmon DNA 的配制: 组份浓度10 mg/ml Salmon DNA ;配制量约100 ml 配制方法: 1. 称取鲑鱼精DNA 2 g置于500 ml烧杯中,加入约200 ml的TE Buffer。 2. 用磁力搅拌器室温搅拌2~4小时
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
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