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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
根据瓶身LOT号查询
- 英文名:
Mineral oil
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
8042-47-5
- 规格:
500ML
属性
产品名称
矿物油, light oil, (neat)
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
sterility
non-sterile
form
light oil
storage condition
protect from light
refractive index
n20/D 1.467 (lit.)
density
0.84 g/mL at 25 °C (lit.)
format
neat
Quality Level
200
Analysis Note
Application
Disclaimer
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文献和实验Placental miR-340 mediates vulnerability to activity based anorexia in mice.
Anorexia nervosa (AN) is a devastating eating disorder characterized by self-starvation that mainly affects women. Its etiology is unknown, which impedes successful treatment options leading to a limited chance of full recovery. Here, we show that gestation is a vulnerable window that can influence the predisposition to AN. By screening placental microRNA expression of naive and prenatally stressed (PNS) fetuses and assessing vulnerability to activity-based anorexia (ABA), we identify miR-340 as a sexually dimorphic regulator involved in prenatal programming of ABA. PNS caused gene-body hypermethylation of placental miR-340, which is associated with reduced miR-340 expression and increased protein levels of several target transcripts, GR, Cry2 and H3F3b. MiR-340 is linked to the expression of several nutrient transporters both in mice and human placentas. Using placenta-specific lentiviral transgenes and embryo transfer, we demonstrate the key role miR-340 plays in the mechanism involved in early life programming of ABA.
:Formerly listed as Type III 溶解性:溶于水,参考浓度2mg/ml 储存条件:2-8℃ From Sigma D1626 Salmon DNA 的配制: 组份浓度10 mg/ml Salmon DNA ;配制量约100 ml 配制方法: 1. 称取鲑鱼精DNA 2 g置于500 ml烧杯中,加入约200 ml的TE Buffer。 2. 用磁力搅拌器室温搅拌2~4小时
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
ES and TS cell freezing/thawing
so as to get them into a homogenous suspension. 6. Then add 100 microliter 2x cell freezing media to the wells, and again pipette to mix. 7. Finally add 80-100 microliter sterile mineral oil (Sigma, Cat. No. M-8410) to cover the cell/freezing medium mixture
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