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文献和实验The Sophisticated Mouse: Protecting a Precious Reagent
Definable, genetically and environmentally, the humble mouse has become a reagent with which to probe the human condition. The information thus gained is leading to a greater understanding of inter-individual variation in drug responses
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
Chloroform: Fresh choloroform + 1/20 vol. isoamyl alcohol. Absolute ethanol. Procedure: 1) Cut 3 mm of toe or tail or toe tips from 7-10 day old mice into 1.5 mL microcentrifuge tubes. 2) Digest tail biopsy by adding 0.7 mL lysis buffer
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