StrongBlock Buffer有三种类型:Strong Block I,Strong Block II 和Strong Block III。StrongBlock I 含有物理改性和化学修饰的酪蛋白及一种专利成分; StrongBlock II 含有物理改性和化学修饰的牛血清白蛋白及其它两种专利成分;StrongBlock III 含有物理改性和化学修饰的牛血清白蛋白,异性抗体封闭剂及一种专利成分。
实验设计:
Block the microplate well by different blocking reagents
Wash the plates 5 times using 0.5% PBST
Load 25µL of pembrolizumab (1 µg/mL) into corresponding microplate wells
Incubate 2 hours at 600 rpm and ambient temperature
Collect the pembrolizumab solution for electrophoresis and BCA test
实验数据:
高效封闭 1)StrongBlock I-III demonstrates better blocking performance than BSA, Casein and skim milk. 2)35-50% blocking efficiency improvement