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- 详细信息
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- 供应商:
上海益启生物科技有限公司
- 规格:
1EA
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文献和实验dishes and allow it to set. After the gel has set, use a narrow cork-borer to punch small cylinders in the gel. Then, using a mounted needle, remove each of these cylinders to create a series of similar sized wells in the agar
Wholemount Immuno-flurescentWholemount Immuno-flurescent
as much extraembryonic membranes as possible. Puncture the amnion and in older embryos (>E10) puncture the head with a syringe or tungsten needle. 2. Fix in 4% paraformaldehyde (PFA) in PBS at 4o C overnight. 3. Wash twice for 5-10 minutes in PBS. 4. Wash
membranes as possible. Puncture the amnion and in older embryos (>E10) puncture the head with a syringe or tungsten needle. 2. Fix in 4% paraformaldehyde (PFA) in PBS at 4oC overnight. 3. Wash twice for 5-10 minutes in PBS. 4. Wash with 50% MeOH/PBS
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