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- 2025年12月31日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海益启生物科技有限公司
- 规格：
100 ML
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文献和实验-3’ (in polII-neo) 3’ oligo: (JC48): 5’-GAA ATC CAG TCC TGC AGT ACA GCT GC-3’ PCR Conditions... Boehringer Mannheim Taq Polymerase and 10X BM PCR buffer with DMSO. PCR program: 95 C x 3’; 30 x (95 C x 1’ 55 C x 1’ 72 C x 3’); 72 C x 3’ Product
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
for use with TMB as the substrate (cover wells with clingfilm during incubations to prevent evaporation). 1) Add 3ul H2O2 to 20ml of Citrate/Acetate buffer. To every 5ml of buffer add 50ul TMB solution. N.B.: Colour change is from colourless to blue
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
in rabbits.10) Wash 4x with 1x PBS containing 0.01% tween.11) Add substrate (100ul/well).12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells should turn blue.13) Stop reaction by the addition of 25ul 0.1M H2SO4/well.14) Read
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