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文献和实验Immobilization of Enzymes on Magnetic Particles
Magnetic particles have been increasingly used as carriers for binding proteins, enzymes, and drugs. Such immobilization procedures for proteins, enzymes, antibodies, and other biologically active compounds have a major impact in different
Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab)2, and Streptavidin
Colloidal gold probes, including protein A-, IgG-F(ab’ )2 -, and streptavidin-labeled gold particles, are useful tools for localization of antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different
Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads
during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobilized double-stranded DNA is converted into a single
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