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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海益启生物科技有限公司
- 规格:
4 X 1250 μL
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文献和实验. It is very important to have equal concentrations of each dNTP (dATP, dCTP, dGTP, dTTP), as inaccuracy in the concentration of even single dNTP dramatically increases the misincorporation level. When maximum fidelity of the PCR process is crucial, the final dNTP
PCR Method to Make Radioactive Probes
-salt 0.02% (w/v) SDS 50% Formamide Blot Wash 1: 2X SSC 0.1% SDS Blot Wash 2: 0.1X SSC 0.1% SDS Method PCR Reaction Combine reaction mix on ice:
Freeze Squeeze PCR Product Purification from Agorose Gel
Abstract: The “Freeze squeeze” method is a cost effective and technically simple procedure to isolate and prepare PCR products for DNA sequencing. Reagents •70% ethanol •3 M sodium acetate Procedure 1.Run your PCR product of interest on a 1
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