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- Sigma-Aldrich
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- F4021-2UG
- 2025年07月09日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
−20°C
- 保质期：
根据瓶身LOT号查询
- 英文名：
Follicle Stimulating Hormone from human pituitary
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
9002-68-0
- 规格：
2UG
属性
生物来源
human pituitary glands
质量水平
200
无菌性
non-sterile
表单
powder
比活
~7,000 IU/mg (powder)
分子量
35 kDa
技术
cell culture | mammalian: suitable
杂质
HBsAg, HCV and HIV-1/HIV-2, tested negative
pH值（酸碱度）
7.4
溶解性
water: ~50 g/L
UniProt登记号
P01225
P23945
运输
ambient
储存温度
−20°C
基因信息
human ... FSHB(2488) , FSHR(2492)
一般描述
研究领域：细胞信号传导
应用
- 猕猴颗粒细胞的体外黄化
- B黄体细胞增殖检测
- 来自小鼠的腔前卵泡细胞的培养
- 未成熟卵丘卵母细胞复合物（COC）的体外熟化，来自小鼠和
- RAW264.7细胞系的培养物
生化/生理作用
包装
其他说明
质量
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- 作者
- 内容
- 询问日期
文献和实验Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control.
In vitro spermatogenesis (IVS) using air-liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we challenged IVS of rats with modifications of culture medium, by supplementing chemical substances, including hormones, antioxidants, and lysophospholipids. In addition, reducing oxygen tension by placing tissues in an incubator of lower oxygen concentration and/or applying silicone cover ceiling on top of the tissue were effective for improving the spermatogenic efficiency. Through these modifications of the culture condition, rat spermatogenesis up to round spermatids was maintained over 70 days in the cultured tissue. Present results demonstrated a significant progress in rat IVS, revealing conditions commonly favorable for mice and rats as well as finding rat-specific optimizations. This is an important step towards successful IVS in many animal species, including humans.
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