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文献和实验dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
. Alonso M, Martinez A. (2004) GSK-3 inhibitors: discoveries and developments. Curr Med Chem. 11 , 755–763. 15. Stambolic V, Ruel L, Woodgett JR. (1996) Lithium inhibits glycogen synthase kinase-3 activity and mimics wingless signalling
µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore
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