- ￥6600
- GeneTex
- 美国
- GTX11314
- 2025年07月16日
- WB, ICC/IF, ELISA
- Mouse
- Human, Rat, Bovine, Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype III coupled to BSA
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat, Bovine, Pig
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX11314
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, ELISA
- 浓度：
Batch dependent(Please refer to the vial label for the specific concentration.)
- 靶点：
beta Tubulin 3/ Tuj1
- 抗体英文名：
beta Tubulin 3/ Tuj1 antibody [SDL.3D10]
- 抗体名：
beta Tubulin 3/ Tuj1 抗体 [SDL.3D10]
- 规格：
100 μl
ICC/IF analysis of SH-SY-5Y cells using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10] at 1:400(pink) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.
WB analysis of (1)U87 (2) MDBK (3) rat2 cell lysate using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10] at 1:400 .
WB analysis of rat brain cytosol extract using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10].
Lane A : Antibody dilution 1:400
Lane B : Negative control (only secondary antibody)
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文献和实验Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
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