相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Liquid Sample Total Cholesterol(TC)Content Assay Kit
- CAS号:
//
- 保质期:
6个月
- 供应商:
普利莱
- 保存条件:
4°保存
- 规格:
125次/250次
| 规格: | 125次 | 产品价格: | ¥660.0 |
|---|---|---|---|
| 规格: | 250次 | 产品价格: | ¥960.0 |
描述:血浆总胆固醇包括2/3的胆固醇酯和1/3的游离胆固醇,是血脂的重要组分,二者与心脑血管疾病以及多种病理状态密切相关。本试剂盒采用世界卫生组织(WHO)、美国FDA、中国《全国临床检验操作规程》推荐的总胆固醇和游离胆固醇检测方法,灵敏度高,检测范围20~5000 µmol/L。可靠性和重复性俱佳。
原理:本试剂盒测定方法采用CHOD-PAP终点方法结合经典GPO Trinder酶学反应【1】【2】,其原理为:(1) 胆固醇酯酶分解胆固醇酯为游离胆固醇。(2) 胆固醇氧化酶将游离胆固醇氧化,产生过氧化氢。(3) 在过氧化物酶催化下生色底物转化为亚胺,光密度值与胆固醇浓度成正比。
北京普利莱基因技术有限公司坐落在北京中关村高新技术园区,由数名具有精深的学术研究能力和产品开发经验的留美博士创建,先后获得国家及中关村高新技术企业认证,依靠世界领先的技术和理念为支撑,致力于打造生命科学研究领域高品质生物试剂产品和技术服务,成为有国际影响力的、对生命科学研究和人类健康有巨大推动作用的生物技术公司。
经过十几年积累和技术攻关,普利莱以国际产品为标准,全面完善生产流程和产品质量控制体系,并利用自身优势不断整合国内外资源,相继推出数百多种具有自主知识产权的科研试剂和试剂盒。产品范围由创建初期的蛋白质研究试剂,扩展到细胞生物学、分子生物学、生物化学、免疫学和实验医学等各类研究领域。建立了基因检测、蛋白印迹、免疫组化、基因克隆与表达、抗体制备和各类生化指标检测服务平台。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验- Cai D, Jia Y, Lu J, et al. Maternal dietary betaine supplementation modifies hepatic expression of cholesterol metabolic genes via epigenetic mechanisms in newborn piglets[J]. British journal of nutrition, 2014, 112(9): 1459-1468.
- Zhang Q, Hu J, Wu Y, et al. Rheb (ras homolog enriched in brain 1) deficiency in mature macrophages prevents atherosclerosis by repressing macrophage proliferation, inflammation, and lipid uptake[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2019, 39(9): 1787-1801.
- Idriss A A, Hu Y, Sun Q, et al. Fetal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in offspring cockerels with modification of promoter DNA methylation[J]. Poultry science, 2020, 99(5): 2533-2542.
- Idriss A A, Hu Y, Hou Z, et al. Dietary betaine supplementation in hens modulates hypothalamic expression of cholesterol metabolic genes in F1 cockerels through modification of DNA methylation[J]. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2018, 217: 14-20.
- Mao F, Wang M X, Hou X, et al. NTCP deficiency causes gallbladder abnormalities in mice and human beings[J]. Cellular and Molecular Gastroenterology and Hepatology, 2021, 11(3): 831.
- Li K, Li H, Zhang K, et al. Orphan nuclear receptor RORγ modulates the genome-wide binding of the cholesterol metabolic genes during mycotoxin-induced liver injury[J]. Nutrients, 2021, 13(8): 2539.
- Hu Y, Sun Q, Li X, et al. In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks[J]. PloS one, 2015, 10(4): e0122643.
- Cai D, Yuan M, Liu H, et al. Maternal betaine supplementation throughout gestation and lactation modifies hepatic cholesterol metabolic genes in weaning piglets via AMPK/LXR-mediated pathway and histone modification[J]. Nutrients, 2016, 8(10): 646.
- Guo J, Li Y, Yuan Y, et al. Cholesterol-lowering activity of 10-gingerol in HepG2 cells is associated with enhancing LDL cholesterol uptake, cholesterol efflux and bile acid excretion[J]. Journal of Functional Foods, 2022, 95: 105174.
- Liu J, Duan Y, Hu Y, et al. Exogenous administration of chronic corticosterone affects hepatic cholesterol metabolism in broiler chickens showing long or short tonic immobility[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2016, 191: 53-58.
- Zhao M, Huang X, Cheng X, et al. Ketogenic diet improves the spatial memory impairment caused by exposure to hypobaric hypoxia through increased acetylation of histones in rats[J]. PLoS One, 2017, 12(3): e0174477.
- Hu Y, Feng Y, Ding Z, et al. Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes[J]. Poultry science, 2020, 99(6): 3111-3120.
- Hu Y, Sun Q, Zong Y, et al. Prenatal betaine exposure alleviates corticosterone-induced inhibition of CYP27A1 expression in the liver of juvenile chickens associated with its promoter DNA methylation[J]. General and comparative endocrinology, 2017, 246: 241-248.
- Yu C, Liu S, Niu Y, et al. Exercise protects intestinal epithelial barrier from high fat diet-induced permeabilization through SESN2/AMPKα1/HIF-1α signaling[J]. The Journal of nutritional biochemistry, 2022, 107: 109059.
- Zhao S, Li J, Wang L, et al. Pomegranate peel polyphenols inhibit lipid accumulation and enhance cholesterol efflux in raw264. 7 macrophages[J]. Food & function, 2016, 7(7): 3201-3210. E1015E1016
- Zhang B, Zhao S, Yang D, et al. A novel G protein-biased and subtype-selective agonist for a G protein-coupled receptor discovered from screening herbal extracts[J]. ACS Central Science, 2020, 6(2): 213-225.
- Yuan F, Hou L, Wei L, et al. Fowl Adenovirus serotype 4 induces hepatic steatosis via activation of liver X receptor-α[J]. Journal of Virology, 2021, 95(6): e01938-20.
- Chen Y, Wu X, Zhang J, et al. Amino acid starvation‐induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance[J]. EMBO reports, 2022, 23(3): e53373.
- Li H, Huang Z, Zeng F. Opuntia dillenii Haw. Polysaccharide Promotes Cholesterol Efflux in THP-1-Derived Foam Cells via the PPARγ-LXRα Signaling Pathway[J]. Molecules, 2022, 27(24): 8639.
2~7.4℃在细胞生长过程中,随细胞数量的增多和代谢活动的加强,二氧化碳不断被释放,培养液变酸,PH值发生变化。由于NaHCO3容易分解为二氧化碳,很不稳定,致使缓冲系统难以精确地控制。故这一缓冲系统适合密闭培养。HEPES结合碳酸氢钠使用,可提供更有效的缓冲体系,主要是防止pH值迅速变动,但最大缺点是在开放培养或观察时难以维持正常的pH值。造成pH波动主要是代谢产生的CO2,在封闭式培养过程中CO2与水结合产生碳酸,培养基pH很快下降。为解决这一问题,合成培养基中使用了NaHCO3-CO2缓冲
技术资料暂无技术资料 索取技术资料










