- 询价
- Tanda
- TD-AB0018
- 中国
- 2025年12月17日
- WB
- Mouse
- Plants
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Plant actin mouse monoclonal antibody
- 抗体英文名：
Plant actin mouse monoclonal antibody
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
Plants
- 保质期：
1年
- 抗原来源：
Mouse
- 级别：
科研级
- 库存：
800
- 供应商：
武汉天德
- 克隆性：
单克隆
- 保存条件：
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
- 形态：
液体
- 亚型：
IgG1
- 免疫原：
Fusion protein of Nicotiana tabacum ACTIN
- 规格：
100μl
Plant actin mouse monoclonal antibody
Cat.# TD-AB0018
Package 25 μl/100 μl/200 μl
Storage PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Product Imformation
Description A nti-plant-actin mouse monoclonal antibody
Applications WB
Immunogen Fusion protein of Nicotiana tabacum ACTIN
Reactivity Plants
Host species Mouse
Ig class IgG1
Purity Protein A/G Affinity Purification
Target information
Symbol plant-actin
Full name Actin
Target Background
Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.
Western blotting
Predicted band size 42 kDa
Positive control Corn leaves lysate, Rice leaves lysate and Nicotiana tabacum leaves lysates
Recommended dilution 500-2000
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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