- ￥4800
- Tanda
- 美国
- TD-26906
- 2025年10月20日
- IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant full length protein of active Gαs
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白表达
- 目录编号：
TD-26906
- 级别：
科研级
- 库存：
88
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
- 浓度：
1mg/ml
- 靶点：
Gαs
- 抗体英文名：
Anti-Gαs-GTP Mouse Monoclonal Antibody
- 抗体名：
Gαs-GTP
- 规格：
30 μL
Gαs-GTP
| Cat. #: TD-26906 |
| Size: |
| Gene Symbol: Gnas |
| Description: Anti-Gαs-GTP Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαs represents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases. |
| Immunogen: Recombinant full length protein of active Gαs |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Gαs antibody recognizes active Gαs of vertebrates |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Gαs Activation Assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti Gαs-GTP monoclonal antibody (Cat. No. TD-26906). Immunoblot was with an anti- Gαs monoclonal antibody (Cat. No. ). |
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文献和实验preparations, GTP can be substituted by 35 S-labeled guanosine 5′-O -(3-thio)triphosphate ([35 S]GTγS) and on agonist stimulation a stable [35 S]GTPγS-Gα complex will form and accumulate. Separation of 35 S-bound GTPγS-Gα complexes from free [35 S]GTPγS allows
甾类（化合物）17α-羟化酶 steroid 17α－hy- droxylase，s eroid 17α-monooxygeoase
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Measurement of GTP-Binding and GTPase Activity of Heterotrimeric G Proteins
activities of Gα protein, which binds and hydrolyses GTP. Evaluation of the rate of GTP binding, the rate of GTP hydrolysis, and the rate of GTP/GDP exchange on Gα protein are required to better understand the mechanistic aspects of heterotrimeric G-protein
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