- ￥4800
- Tanda
- 美国
- TD-26231
- 2025年10月26日
- ELISA, WB, IF, IHC
- Mouse
- 脊椎动物
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of IDH2 which includes the mutation of R172G, human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26231
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IF, IHC
- 浓度：
1mg/ml
- 靶点：
R172G
- 抗体英文名：
Anti-IDH2 (R172G) mouse monoclonal antibody
- 抗体名：
IDH2(R172G)
- 规格：
100 μL
IDH2(R172G)
| Cat. #: TD-26231 |
| Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| Description: Anti-IDH2(R172G) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172G IDH2 mutations do exist in diffusely infiltrative gliomas. |
| Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172G, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes R172G mutant, but not wild type IDH2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant IDH2(R172G) and wild type proteins. Purified His-tagged IDH2(R172G) (lane 1) and corresponding wild type IDH2 protein (lane 2) were blotted with Anti-IDH2(R172G) monoclonal antibody (Cat. #TD-26231). Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172G) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172G) plasmid (right column), then fixed and stained with Anti-IDH2(R172G) monoclonal antibody (Cat. #TD-26231). |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验一般R250 染蛋白，G250 一般测蛋白浓度，也可染胶，敏感性更高但较慢脱色较难。 G250常用的蛋白染料，作定性试验用,染色后为蓝绿色;R250较敏感,，做定量实验用,染胶效果较好.染色后为红蓝色 考马斯亮蓝R250（Coomassie brilliant blue R250）。C45H44O7H3S2Na，MW=824，λmax
攻克「癌症之王」！哈佛大学卢坤平团队提出消除肿瘤新方法，这个
-L1 的溶酶体降解 [5]，同时 HIP1R 蛋白还存在 Pin1 的潜在底物识别位点。进一步的机制研究发现，Pin1 可能通过作用于 HIP1R 中的 pSer929-Pro 位点来促进肌动蛋白结合，进而促进 PD-L1 和 ENT1 的溶酶体降解过程。图片来源：Cell为了确定 Pin1 抑制剂是否能够破坏免疫抑制的肿瘤微环境，并通过与免疫化疗相结合根除原发性 PDAC 肿瘤，研究人员使用了在胰腺中发生 KrasG12D 和 p53R172H 突变的 KPC 小鼠。这些小鼠表现出 PDAC 肿瘤的异质
in planta tra n sfo rm a tio n m eth o d f o r w h ea t (Triticumaestivum L. ) u sin g Agrobacterium tumefaciens. J. Biosci. Bioeng. 102, 162-170. 12. Holme, I. B., Brinch-Pedersen, H ., Lange, M. and Holm, P. B.
技术资料暂无技术资料 索取技术资料
