Description Basic Fibroblast Growth Factor (bFGF) is a member of the FGF family of mitogenic peptides which is comprised of at least 23 proteins. FGF signaling factors play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. It was originally named bFGF based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Synonyms FGF-2, Fibroblast Growth Factor basic (FGFb) AA Sequence MTMITNSSSV PGDPLESMAA GSITTLPALP EDGGSGAFPP GHFKDPKRLYCKNGGFFLRI HPDGRVDGVR EKSDPHIKLQ LQAEERGVVS IKGVCANRYLAMKEDGRLLA SKCVTDECFF FERLESNNYN TYRSRKYSSW YVALKRTGQYKLGPKTGPGQ KAILFLPMSA KS Source Escherichia coli Molecular Weight Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 172 amino acids Biological Activity Fully biologically active. The ED50 is < 1.0ng/ml, corresponding to a specific activity of 1.0 Physical Appearance White lyophilized powder. Formulation Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, containing 4% mannitol. Endotoxin < 1EU/μg of growth factor as determined by LAL method. Reconstitution Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Storage Store at -20°C after receiving. Upon Reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. Usage This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.