- 询价
- 北纳创联
- ATCC PRA-273
- 美国
- 2025年10月15日
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- 详细信息
- 技术资料
- 供应商：
北纳创联
- 保质期：
1-2年
- 英文名：
Thraustochytriidae
ATCCPRA-273 thel-2
| 基本信息 | |
| 资源编号 | ATCCPRA-273 |
| 资源名称 | thel-2 |
| 种属 | Thraustochytriidae g. sp. |
| 分离基物 | Marine invertebrate gut contents Isolated by W Marshall, 2004 Bamfield Inlet, British Columbia, Canada |
| 提供形式 | frozen |
| 安全等级 | 1 |
| 模式菌株 | no |
| 培养方法 | |
| 培养基 | Medium 2673: Thraustochytrid medium |
| 生长条件 | Growth condition: axenic Temperature: 15.0°C |
| 存储条件 | 1. To achieve the best results set up cultures with several different inocula (e.g. 0.25 ml, 0.5 ml, 1.0 ml). Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. 2. If the cell concentration exceeds the required level do not centrifuge, but adjust the concentration to between 2 x 106 and 2 x 107cells/ml with fresh medium. If the concentration is too low, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield the desired concentration. 3. While cells are centrifuging prepare a 20% (v/v) solution of sterile DMSO as follows: Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath. Allow the DMSO to solidify. Add the required volume of refrigerated medium. Dissolve the DMSO by inverting the tube several times. *NOTE: If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium. 4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 106 and 107 cells/ml and 10% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 30 min. 5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6. Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.) 7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer. 8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial. 9. Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 2673 in a T-25 tissue culture flask or 16 x 125 mm screw-capped test tube. Incubate at 15°C. |
| 详细说明 | |
| 描述 | Unclassified Thraustochytriidae Phylogenetic study |
| Year of Origin | 2004 |
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北纳创联thel-2
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