Description Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic
stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Synonyms D-FACTOR, differentiation-stimulating factor AA Sequence MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F Source Escherichia coli Molecular Weight Approximately 20 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. Biological Activity Activity determined by its ability to induce differentiation of murine M1 myeloid leukemic cells. Minimum detectable concentration in assay is 0.5ng/mL, corresponding to specific activity > 1 x 10^8 u Physical Appearance White lyophilized powder. Formulation Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, with 0.02% TWEEN 20. Endotoxin < 1EU/μg of growth factor as determined by LAL method. Reconstitution Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Storage Store at -20°C after receiving. Upon Reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. Usage This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.