Description VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
Molecular Weight Approximately 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
Biological Activity Fully biologically active. The ED50 is 1-8ng/ml, as determined by HUVEC cell proliferation.
Physical Appearance White lyophilized powder.
Formulation Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin < 1EU/μg of growth factor as determined by LAL method.
Reconstitution Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
Storage Store at -20°C after receiving. Upon Reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles.
Usage This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.