重组人血管内皮生长因子165（VEGFA）

重组人血管内皮生长因子165（VEGFA）

PRP100240 | Human VEGF165 protein

---

▎关键参数

• 来源表达：人源细胞表达系统，无标签蛋白
• 生物活性：HUVEC细胞增殖ED₅₀=4-16 ng/ml（实测值）
• 纯度验证：还原性SDS-PAGE显示>95%，双条带（20/22 KDa）
• 内毒素：＜0.1 EU/μg（LAL法）

▎产品特性

• 天然构象：基于人源VEGF165 isoform（Met1-Arg191）序列构建
• 糖基化特征：保留天然糖基化修饰，与猕猴序列100%同源
• 制剂工艺：冻干缓冲液含100 mM Glycine+10 mM NaCl（pH7.0）

▎使用指南

• 复溶建议：使用原装复溶Buffer，终浓度≥100 μg/mL
• 保存方案：
  • 冻干粉：-20℃以下长期保存
  • 溶解后：4℃保存≤7天，-20℃长期保存需添加0.1%载体蛋白
• 实验前处理：开盖前离心，避免反复冻融

---

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Vascular Endothelial Growth Factor 165 (VEGFA)

PRP100240 | Human VEGF165 protein

---

▎Key Parameters

• Expression System:Human cell expression system, tag-free protein
• Biological Activity:HUVEC cell proliferation ED₅₀=4-16 ng/ml (measured value)
• Purity Verification:>95% by reducing SDS-PAGE, double bands (20/22 KDa)
• Endotoxin:＜0.1 EU/μg (LAL method)

▎Product Characteristics

• Native conformation: Constructed based on human VEGF165 isoform (Met1-Arg191) sequence
• Glycosylation profile: Retains natural glycosylation modifications, 100% homologous to macaque sequence
• Formulation: Lyophilized buffer containing 100 mM Glycine+10 mM NaCl (pH7.0)

▎Usage Guidelines

• Reconstitution:Use original reconstitution Buffer, final concentration ≥100 μg/mL
• Storage:
  • Lyophilized powder: Long-term storage below -20℃
  • Reconstituted solution: ≤7 days at 4℃, long-term storage at -20℃ requires 0.1% carrier protein
• Pre-experiment handling:Centrifuge before opening, avoid repeated freeze-thaw cycles

---

Note: The above information may change due to product optimization and upgrades. For specific details, please consult AcroBiosystems.