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Tough-Tags® Large, Blue
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文献和实验large fragment cloning-Molecular Biology
, and none of them all of it. Can pBlueScript realistically take inserts of 12.5kb or would I be better off trying a different vector e.g. a phagemid or cosmid? Does anybody have any tips for dealing with large inserts generally? I use XL-10 gold or XL-1 blue cells for the transformation
Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition
Inflammasomes are large cytosolic multi-protein complexes that form in response to infection and other stimuli, and play an important role in the innate immune response. Traditional methods for assaying inflammasome activation have relied
and adjacent proteins. However, this can result in large amounts of nonspecific damage to bases not actually involved in crosslinking; this limits, e.g., the ability to subsequently map the sites involved in crosslinking. More refined approaches involve
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