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- 文献和实验
- 技术资料
- 英文名:
ChromaFlash One-Step Magnetic ChIP Kit
- 供应商:
上海西宝
- 规格:
48次样本
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文献和实验were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated
Mag-Binds Plant DNA Kit Magnetic Fast Protocol
during the transfer in next step. 5. Carefully transfer 400 ul supernatant to 2ml Deep Well Plate, making sure not to disturb the pellet or transfer any debris. 6. Add 20 ul MagSi Particles and 280 ul absolute thanol to each well. Shaking to mix for 2 min
Mag-Binds Plant DNA Kit Magnetic 96 Protocol
may float. Be careful to avoid those particles during the transfer in next step. 4. Carefully transfer 400 ul supernatant to 2ml Deep Well Plate, making sure not to disturb the pellet or transfer any debris. 5. Add 20 ul MagSi Particles and 280
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