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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海西宝
- 英文名：
Deoxyribonucleic Acid, Denatured, Fragmented (SDNAD)
- 规格：
Bulk
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham) basically by the method of Feinberg and Vogelstein (9 ). DNA Labeling Kit (Takara
Methods for the Detection of D-Amino-Acid Oxidase
phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham
, because, as the peptides elute from the column, they are directed into the ESI ion-trap mass spectrometer, where they are ionized, mass-selected, and fragmented. Finally, advanced search algorithms match the fragmented peptides to their respective proteins
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