Deoxyribonucleic Acid, Denatur

Methods for the Detection of D-Amino-Acid Oxidase

/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham) basically by the method of Feinberg and Vogelstein (9 ). DNA Labeling Kit (Takara

Methods for the Detection of D-Amino-Acid Oxidase

phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)

, because, as the peptides elute from the column, they are directed into the ESI ion-trap mass spectrometer, where they are ionized, mass-selected, and fragmented. Finally, advanced search algorithms match the fragmented peptides to their respective proteins