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- 文献和实验
- 技术资料
- 供应商：
上海西宝
- 英文名：
Collagenase Vial, NCIS (CLSPANK)
- 规格：
1 vi
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文献和实验/NCIS/default.html . We have a modified adult rat cardiomyocyte isolation tutorial that we can fax upon request which recommends the Langendorf Perfusion System and Collagenase Type II. 1.2. What are the volumes of Hank's, Earle's and Leibovitz
Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells
pellet (gently) in 3 ml ES media to wash away collagenase. Spin 5 minutes at 1000 rpm. Resuspend pellet (again, gently!) in 0.25 ml Resuspension Medium per vial (This is one half the final volume required for freezing). Dropwise, add
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
β-mercaptoethanol, 1000X Gibco® Mouse Embryonic Fibroblasts (Irradiated) Basic Fibroblast Growth Factor (bFGF) GlutaMAX™-I (100X) Collagenase Type IV for enzymatic passaging or StemPro® EZPassage™ Tool for mechanical
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