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- 详细信息
- 文献和实验
- 技术资料
- 库存:
包
- 供应商:
上海觅拓生物科技有限公司
- 规格:
1 mL
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文献和实验Mouse Embryonic Stem Cell Adherent Cell Differentiation and Cytotoxicity Assay
uses an In-Cell Western technique after a 9-day culture. DRAQ5/Sapphire700 cell/DNA stains are used to quantify cell number and myosin heavy chain (MHC) protein is used as a marker of cardiomyocyte differentiation. MHC is corrected for cell number
Live imaging with Drosophila tissue culture cells
-terminal fusions and in general are non-toxic to the cells. FP's fused to histones (e.g. Histone H2B) are a good alternative for a chromosome counter stain. Hoechst 33342 and Draq5 are DNA specific live dyes, that don't stain significantly
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
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