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- 详细信息
- 文献和实验
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- 保质期:
2019/11/1
- 规格:
支
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文献和实验1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00) 50 mM Tris pH7.8 40 g ammonium sulfate for every 100 ml
preparation (serum, tissue culture supernatant, or ascites). Apply Binding buffer again ~ 200 ml. Apply Elution buffer ~ 100 ml. Collect 3 ml fractions in 5 ml tubes with 700 ul 1 M Tris pH 9.0 already in bottom of tube to neutralize
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