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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海博湖
- 检测范围:
7.8-500ng/ml
- 检测方法:
Sandwich ELISA, Double Antibody
- 应用:
4.688ng/ml
- 样本:
VTCN1
- 规格:
96T
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文献和实验for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
Activation and Expansion of Human Treg Cells
1: PBS w/ 0.1% BSA, pH 7.4 Cultural Medium: OpTmizer? T-Cell Expansion SFM (Gibco) serum free 1X formulation designed to support the culture and expansion of human T cells(or equivalent) Round bottom tissue culture plates or tissue culture flasks
the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
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