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文献和实验for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
v468 Chapter 9 自身启动子报告基因验证转录靶点
of the canonical Wnt signaling pathway.However, b-catenin does not itself bind DNA, but functions via interaction with T-cell factor (TCF)/lymphoid-enhancing factor (LEF) transcription factors. These proteins contain a high-mobility group(HMG) box that binds DNA
Introduction of human 14-3-3 proteins
-1,was markedly increased following T cell activation.The use of truncated 14-3-3tau fusion proteins demonstrated that the15℃terminal residues are required for the association between 14-3-3 and three of its target proteins,namely,Cbl,Raf-1,and PI3-K.The findings
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