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Fixed Speed Peristaltic Pump AssemblyProvides constant flow of 80–100 mL/min (depending on AC frequency) |
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Peristaltic PumpsA variable-speed (60–600 RPM) pump drive allows flow rates ranging from 480 to 1,680 mL/min, depending on pump head selected. Pump heads must be ordered separately. For higher flow rates up to 13 L/min, an Easy-Load pump, complete with pump head, is available. |
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详细描述见链接:http://www.millipore.com/catalogue/item/XX80EL004
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文献和实验by centrifugation x 5 min at 600g and resuspend in 5 ml of EPC medium. 6. Cell seeding of slide prior to flow chamber assembly 1) A slide made out of glass, titanium, or other material can be used, and the slide surface can be modified by spin
Plant DNA methylation analysis by bisulfite genomic sequencing
(see comment 3); Centrifuge Sigma 1-15K (or equivalent) >15 minutes at 4°C 12000rpm (12000 X g); Discard supernatant, add 1.0ml 70% ethanol (room temperature), vortex; Centrifuge Sigma 1-15K (or equivalent) >15 minutes at 4°C 12000rpm
Bromodeoxyuridine Immunohistochemistry
between the following steps, and use apx. 20 ml for each reagent (step 3-7). 6. 30, 50, 70, 95, 100 x 2, RT, 30 min. each , with slight agitation. This step functions to dehydrate the tissues through an increasing ethanol series: Samples in 70, 95, or 100
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