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- 2025年07月16日
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文献和实验Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organism from which you buy secondary antibodies, Jackson Immunolabs
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
withPBS, maintain the cells in 25 or 30 mL of serum-free DMEM,and collect conditioned media every 3 days for five to sevenharvests. MDCK monolayers can be cycled from serum-free toserum-containing DMEM for 3-day intervals to prolong cellviability
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
, Millipore) Microbalance (e.g., Sartorius ME36S) 10‐ 50‐, and 100‐ml narrow‐necked volumetric flasks
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