- 询价
- S26-100ML
- 2025年07月06日
- Inhibits Activity/Function
- 0
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- 应用范围：
Inhibits Activity/Function
- 宿主：
0
- 库存：
大量
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0
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0
- 规格：
100 mL
HEMOGLOBIN: ~3.5 mg/dL
COUNTRY OF ORIGIN: USA
Optimal dilutions determined by enduser.
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
well inserts and cut out mesh insert with a scalpel, forceps and dissecting microscope Place inserts in a 2.0 mL Eppendorf tube with 1 mL of blocking agent (10% goat serum, 1% BSA in PBS). Gently flick tube to mix. Remove solution and wash 3x
Antibody Staining of Dissected Gonads
in the dissecting scope it is tedious. Materials PBS -- Sambrook et al. Molecular Cloning book. PBS/0.2 mM Levamisole (from 100 mM Levamisole stock). PBS/0.1% Tween 20 (PBTw) Blocking buffer; 30 % goat serum in PBTw or 1 mg/ml BSA in PBTw.
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
mM NaCl = 30ml 5M NaCl 50mM Tris-HCl, pH 7.5 = 50ml 1M Tris-HCl 0.1 BSA = 10ml 10 BSA H2O to 1L 0.1 Triton X-100 1ml 100 Triton X-100 0.05 Thimerosal = 0.5g Thimerosal (Hg-containing poison!) TBS + N TBS + 2 appropriate serum (e.g
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