白介素试剂盒

ELISA 原理
酶联免疫吸附试验 (Enzyme Linked Immunosorbent Assay，ELISA) 是目前应用比较广泛的免疫学检测技术，是将抗原 - 抗体反应的特
异性与酶催化作用的高效性相结合，通过酶作用于底物后的显色反应判定结果。一般用酶标测定仪测定吸光度（OD 值）来反映抗原或抗体含量，灵敏度可达每毫升纳克 (ng) 水平甚至皮克 (pg) 水平。由于酶的催化效率很高，间接地放大了免疫反应的结果，使测定方法达到很高的敏感度。目前常用的 ELISA 方法有直接法、间接法、双抗夹心法、竞争法 ELISA。

样本不同，稀释液不同
针对不同的样本，研发不同稀释液，以降低基质效应干扰，保证结果的可靠性。
筛选特定稀释液，血清和血浆类样本稀释液PT 1-ag，细胞上清类样本稀释液PT4，且加标回收率在80-120%之间 ，样本中 “基质”效应干扰有效排除。
板内板间均一性测定
严格控制每一块板孔之间和不同板之间的变异系数（CV）小于 10%，保证检测结果的一致性。
实验流程
1. 将标准品梯度稀释、待测样本加入酶标板后 37℃孵育 120 min 后用 wash buffer 洗涤 4 次；
2. 加入检测抗体溶液后 37℃孵育 60 min 后用 wash buffer 洗涤 4 次；
3. 加入 HRP 溶液 37℃孵育 40 min 后用 wash buffer 洗涤 4 次；（如果检测抗体已偶联 HRP，则省略此步。）
4. 加入 TMB 底物进入显色步骤，经过 37℃孵育 15-20 min，此时酶标板内溶液显蓝色。
5. 加入 Stop Solution 溶液进行终止，酶标板内液体会由蓝色转为黄色，此时尽快放入酶标仪中读数。
ELISA 试剂盒说明书中指标的意义

当我们的待测样本的浓度 <7.8 pg/mL 时 ？
当待测样本 < 7.8 pg/mL 时，需要考虑说明书中“Sensitivity”这个指标。它指的是免疫检测产品的分析灵敏度，在说明书第
5 页，“Sensitivity”值为 1.2 pg/mL，说明待测样本在 1.2-7.8 pg/mL 范围时是可以检测，但是测定值并不一定准确。当测定值
<1.2 pg/mL，则认为该测定值为稀释液的“0”浓度所在的范围。
那么当我们的待测样本的浓度 >500 pg/mL ？
此时需要对样本进行最佳稀释比的摸索，使其浓度落在标曲范围内。比如 PHA 刺激 PBMC 3 天后细胞上清中样本值在32 ng/mL，那么样本至少需要稀释 64 倍才可以落在标曲范围内。为了找到最佳的稀释比，可以用少量样本进行预实验，同时也可以参考说明书中部分的建议。

Human IFN-α(Interferon Alpha) ELISA Kit	IFN-α
Human IgA1(Immunoglobulin A1) ELISA Kit	IgA1
Human IgA2(Immumoglobulin A2) ELISA Kit	IgA2
Human IGF1R3(Insulin Like Growth Factor 1 Receptor 3) ELISA Kit	IGF1R3
Human IGF2BP3(Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit	IGF2BP3
Human IGF2R(Insulin Like Growth Factor 2 Receptor) ELISA Kit	IGF2R
Human IGFALS(Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) ELISA Kit	IGFALS
Human IgG1(Immunoglobulin G1) ELISA Kit	IgG1
Human IgG2(Immunoglobulin G2) ELISA Kit	IgG2
Human IgG3(Immunoglobulin G3) ELISA Kit	IgG3
Human IgG4(Immunoglobulin G4) ELISA Kit	IgG4
Human IL10Rβ(Interleukin 10 Receptor Beta) ELISA Kit	IL10Rβ
Human IL-13(Interleukin 13) ELISA Kit	IL-13
Human IL-17(Interleukin 17) ELISA Kit	IL-17
Human IL-19(Interleukin 19) ELISA Kit	IL-19
Human IL-23(Interleukin 23) ELISA Kit	IL-23
Human IL-35(Interleukin 35) ELISA Kit	IL-35
Human INHB(Inhibin B) ELISA Kit	INHB
Human INSL5(Insulin Like Protein 5) ELISA Kit	INSL5
Human IRF5(Interferon Regulatory Factor 5) ELISA Kit	IRF5
Human ITLN2(Intelectin 2) ELISA Kit	ITLN2
Human IκBα(Inhibitory Subunit of NF Kappa B Alpha) ELISA Kit	IκBα
Human KERA(Keratocan) ELISA Kit	KERA
Human LACTβ(β-Lactamase) ELISA Kit	LACTβ
Human LAMA4(Laminin Alpha 4) ELISA Kit	LAMA4
Human LAT(Linker For Activation of T-cell) ELISA Kit	LAT
Human LCA/CD45(Leukocyte Common Antigen) ELISA Kit	LCA/CD45
Human LDHB(Lactate Dehydrogenase B) ELISA Kit	LDHB
Human LDL(Low Density Lipoprotein) ELISA Kit	LDL
Human LFA-1(Lymphocyte Function Associated Antigen 1) ELISA Kit	LFA-1
Human LIPH(Lipase H) ELISA Kit	LIPH
Human LOX(Lysyl Oxidase) ELISA Kit	LOX
Human LOXL4(Lysyl Oxidase Like Protein 4) ELISA Kit	LOXL4
Human LTA(lipoteichoic acids) ELISA Kit	LTA
Human LpPLA2(Phospholipase A2, Lipoprotein Associated) ELISA Kit	LpPLA2
Human LTA4H(Leukotriene A4 Hydrolase) ELISA Kit	LTA4H
Human LTC4(Leukotriene C4) ELISA Kit	LTC4
Human LTD4(Leukotriene D4) ELISA Kit	LTD4
Human LT-E4(Leukotriene E4) ELISA Kit	LT-E4
Human LTβ(Lymphotoxin Beta) ELISA Kit	LTβ
Human LTβR(Lymphotoxin Beta Receptor) ELISA Kit	LTβR
Human LZMg(Lysozyme G) ELISA Kit	LZMG
Human M-AChR(Muscarinic Acetylcholine Receptor) ELISA Kit	M-AChR
Human MAG Ab(Anti-Myelin Associated Glycoprotein Antibody) ELISA Kit	MAG Ab
Human MAN1A1(Mannosidase Alpha Class 1A Member 1) ELISA Kit	MAN1A1
Human MANF(Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit	MANF
Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit	MAPK
Human MAU(Microalbuminuria) ELISA Kit	MAU
Human MCSFR(Macrophage Colony Stimulating Factor Receptor) ELISA Kit	MCSFR
Human MCSP(Melanoma Associated Chondroitin Sulfate Proteoglycan) ELISA Kit	MCSP
Human ME20M(Melanoma Associated ME20) ELISA Kit	ME20M
Human MT(Melatonin) ELISA Kit	MT
Human MFAP4(Microfibrillar Associated Protein 4) ELISA Kit	MFAP4
Human MFI2(Melanotransferrin) ELISA Kit	MFI2
Human MGL(Monoacylglycerol Lipase) ELISA Kit	MGL
Human MGST1(Microsomal Glutathione S Transferase 1) ELISA Kit	MGST1
Human MHB(Methemoglobin) ELISA Kit	MHB
Human MHCC/HLA-C(Major Histocompatibility Complex Class I C) ELISA Kit	MHCC/HLA-C
Human MHCG(Major Histocompatibility Complex Class I G) ELISA Kit	MHCG
Human mIgM(Membrane Immumoglobulin M) ELISA Kit	mIgM
Human MIP4α(Macrophage Inflammatory Protein 4 Alpha) ELISA Kit	MIP4α
Human MLCK/MYLK(Myosin Light Chain Kinase) ELISA Kit	MLCK/MYLK
Human MMP-10(Matrix Metalloproteinase 10) ELISA Kit	MMP-10
Human MMP-24(Matrix Metalloproteinase 24) ELISA Kit	MMP-24
Human MMP-25(Matrix Metalloproteinase 25) ELISA Kit	MMP-25
Human MMRN1(Multimerin 1) ELISA Kit	MMRN1
Human MN(Metanephrine) ELISA Kit	MN
Human MRP(Multidrug Resistance-Associated Protein) ELISA Kit	MRP
Human MS(Melatonin Sulfate) ELISA Kit	MS
Human MSR1(Macrophage Scavenger Receptor 1) ELISA Kit	MSR1
Human MT1(Metallothionein 1) ELISA Kit	MT1
Human MT1M(Metallothionein 1M) ELISA Kit	MT1M
Human MT3(Metallothionein 3) ELISA Kit	MT3
Human MTL(Motilin) ELISA Kit	MTL
Human MUC13(Mucin 13) ELISA Kit	MUC13
Human MUC17(Mucin 17) ELISA Kit	MUC17
Human MUC3(Mucin 3) ELISA Kit	MUC3
Human MUC6(Mucin 6) ELISA Kit	MUC6
Human MUSKab(Muscle Skeletal Receptor Tyrosine Kinase antibody) ELISA Kit	MUSKab
Human MYCBP(C-Myc Binding Protein) ELISA Kit	MYCBP
Human MYH10(Myosin Heavy Chain 10, Non Muscle) ELISA Kit	MYH10
Human MYL1(Myosin Light Chain 1, Alkali, Fast Skeletal) ELISA Kit	MYL1
Human NAGase(N-Acetyl Beta-D-Glucosaminidase) ELISA Kit	NAGase
Human NAP(Neutrophil Alkaline Phosphatase) ELISA Kit	NAP
Human NAP3(Neutrophil Activating Protein 3) ELISA Kit	NAP3
Human NEB(Noradrenaline Bitartas) ELISA Kit	NEB
Human NEF3(Neurofilament 3) ELISA Kit	NEF3
Human Neopterin ELISA Kit	Neopterin
Human N-ERC/Mesothelin ELISA Kit	N-ERC/Mesothelin
Human NFE2L2(Nuclear Factor, Erythroid Derived 2 Like 2) ELISA Kit	NFE2L2
Human NFκB(Nuclear Factor Kappa B) ELISA Kit	NFκB
Human NF-κB p65(Nuclear Factor Kappa B p65) ELISA Kit	NF-κB p65
Human NKA(Neurokinin A) ELISA Kit	NKA
Human NKB(Neurokinin B) ELISA Kit	NKB
Human N-MID-OT(N-MID Osteocalcin) ELISA Kit	N-MID-OT
Human NMP-22(Nuclear Matrix Protein 22) ELISA Kit	NMP-22
Human NPYR1(Neuropeptide Y Receptor Y1) ELISA Kit	NPYR1
Human NPYR2(Neuropeptide Y Receptor Y2) ELISA Kit	NPYR2
Human NPYR5(Neuropeptide Y Receptor Y5) ELISA Kit	NPYR5
Human NRG-2(Neuregulin 2) ELISA Kit	NRG-2
Human NRG-3(Neuregulin 3) ELISA Kit	NRG-3
Human NRG-4(Neuregulin 4) ELISA Kit	NRG-4
Human NT-ProANP(N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit	NT-ProANP
Human NTRK2(Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit	NTRK2
Human NTRK3(Neurotrophic Tyrosine Kinase Receptor Type 3) ELISA Kit	NTRK3
Human NTXI(Cross Linked N-telopeptide of Type I Collagen) ELISA Kit	NTXI
Human NUMA1(Nuclear Mitotic Apparatus Protein 1) ELISA Kit	NUMA1
Human NUP62(Nucleoporin 62kDa) ELISA Kit	NUP62
Human OC/BGP(Osteocalcin) ELISA Kit	OC/BGP
Human OCT(Ornithine Carbamoyl Transferase) ELISA Kit	OCT
Human OFQ/N(Orphanin FQ/Nociceptin) ELISA Kit	OFQ/N
Human OLAb(Anti-Oxidized Low Density Lipoprotein Antibody) ELISA Kit	OLAb
Human OLFM4(Olfactomedin 4) ELISA Kit	OLFM4
Human ON(Osteonectin) ELISA Kit	ON
Human OP(Oligomeric Protein) ELISA Kit	OP
Human OTR(Oxytocin Receptor) ELISA Kit	OTR
Human OVA sIgE(Ovalbumin Specific IgE) ELISA Kit	OVA sIgE
Human OVA sIgG(Ovalbumin Specific IgG) ELISA Kit	OVA sIgG
Human OXA(Orexin A) ELISA Kit	OXA
Human OXB(Orexin B) ELISA Kit	OXB
Human PⅢCP(Procollagen Ⅲ C-Terminal ProPeptide) ELISA Kit	PⅢCP
Human PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit	PACAP-38
Human PADI4(Peptidyl Arginine Deiminase Type IV) ELISA Kit	PADI4
Human PAH(Phenylalanine Hydroxylase) ELISA Kit	PAH
Human PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit	PAI2
Human PAQR7(Progestin and AdipoQ Receptor Family Member VII) ELISA Kit	PAQR7
Human PAR2(Protease Activated Receptor 2) ELISA Kit	PAR2
Human PAR3(Protease Activated Receptor 3) ELISA Kit	PAR3
Human PARP4(Poly ADP Ribose Polymerase 4) ELISA Kit	PARP4
Human Pax(Paxillin) ELISA Kit	Pax
Human PCDH15(Protocadherin 15) ELISA Kit	PCDH15
Human PCDHβ16(Protocadherin Beta 16) ELISA Kit	PCDHβ16
Human PCDHγA2(Protocadherin Gamma A2) ELISA Kit	PCDHγA2
Human PCI(Protein C Inhibitor) ELISA Kit	PCI
Human PCIII(Procollagen III) ELISA Kit	PCIII
Human PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit	PCNA
Human PCPE1(Procollagen C Proteinase Enhancer 1) ELISA Kit	PCPE1
Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) ELISA Kit	PDCD1LG1
Human PDCN(Podocin) ELISA Kit	PDCN
Human PDE4D(Phosphodiesterase 4D, cAMP Specific) ELISA Kit	PDE4D
Human PDGF(Platelet-Derived Growth Factor) ELISA Kit	PDGF
Human PDGF-AA(Platelet Derived Growth Factor-AA) ELISA Kit	PDGF-AA
Human PDGFRβ(Platelet Derived Growth Factor Receptor Beta) ELISA Kit	PDGFRβ
Human PDH E1(Pyruvate Dehydrogenase E1) ELISA Kit	PDH E1
Human PDHα(Pyruvate Dehydrogenase Alpha) ELISA Kit	PDHα
Human PDHβ(Pyruvate Dehydrogenase Beta) ELISA Kit	PDHβ
Human PDI(Protein Disulfide Isomerase) ELISA Kit	PDI
Human PDIA2(Protein Disulfide Isomerase A2) ELISA Kit	PDIA2
Human PDIA3(Protein Disulfide Isomerase A3) ELISA Kit	PDIA3
Human PDIA5(Protein Disulfide Isomerase A5) ELISA Kit	PDIA5
Human PDIA6(Protein Disulfide Isomerase A6) ELISA Kit	PDIA6
Human PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit	PDK4
Human PDP(Pyruvate Dehydrogenase Phosphatase) ELISA Kit	PDP
Human pERK(Phospho Extracellular Signal Regulated Kinase) ELISA Kit	pERK
Human PEX2(Peroxisomal Biogenesis Factor 2) ELISA Kit	PEX2
Human PF3(Platelet Factor 3) ELISA Kit	PF3
Human PF4V1(Platelet Factor 4 Variant 1) ELISA Kit	PF4V1
Human PFKM(Phosphofructokinase, Muscle) ELISA Kit	PFKM
Human PFKP(Phosphofructokinase, Platelet) ELISA Kit	PFKP
Human PGAM1(Phosphoglycerate Mutase 1) ELISA Kit	PGAM1
Human PGD2S(Prostaglandin D2 Synthase, Brain) ELISA Kit	PGD2S
Human PGDS(Prostaglandin D Synthase) ELISA Kit	PGDS
Human PGF2α(Prostaglandin F2α) ELISA Kit	PGF2α
Human P-GP(Permeability Glycoprotein) ELISA Kit	P-GP
Human PGRP-S(Peptidoglycan Recognition Protein-S) ELISA Kit	PGRP-S
Human PHB(Prohibitin) ELISA Kit	PHB
Human PHSA(Poly Serium Antigen) ELISA Kit	PHSA
Human PI3K2α(Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide) ELISA Kit	PI3K2α
Human PIK3Cβ(Phosphoinositide-3-Kinase Catalytic Beta Polypeptide) ELISA Kit	PIK3Cβ
Human PIK3Cσ(Phosphoinositide-3-Kinase Catalytic Delta Polypeptide) ELISA Kit	PIK3Cσ
Human pIκBα(Phospho-Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit	pIκBα
Human PK(Pyruvate Kinase, Liver And RBC) ELISA Kit	PK
Human PK2(Prokineticin 2) ELISA Kit	PK2
Human PKC(Protein Kinase C) ELISA Kit	PKC
Human PKCβ1(Protein Kinase C Beta 1) ELISA Kit	PKCβ1
Human PKCδ1(Protein Kinase C Delta) ELISA Kit	PKCδ1
Human PKCζ(Protein Kinase C Zeta) ELISA Kit	PKCζ
Human PKCη(Protein Kinase C Eta) ELISA Kit	PKCη
Human PKP1(Plakophilin 1) ELISA Kit	PKP1
Human PKR1(Prokineticin Receptor 1) ELISA Kit	PKR1
Human PKR2(Prokineticin Receptor 2) ELISA Kit	PKR2
Human PL(Placental Lactogen) ELISA Kit	PL
Human PLA1(Phospholipase A1) ELISA Kit	PLA1
Human PLA2G10(Phospholipase A2, Group X) ELISA Kit	PLA2G10
Human PLA2G12B(Phospholipase A2, Group XII B) ELISA Kit	PLA2G12B
Human PLA2G4D(Phospholipase A2, Group IV D) ELISA Kit	PLA2G4D
Human PLAA(Phospholipase A2 Activating Protein) ELISA Kit	PLAA
Human PLB(Phospholipase B) ELISA Kit	PLB
Human PLC(Phospholipase C) ELISA Kit	PLC
Human PLCγ1(Phospholipase C Gamma 1) ELISA Kit	PLCγ1
Human PLCγ2(Phospholipase C Gamma 2, Phosphatidylinositol Specific) ELISA Kit	PLCγ2
Human PLGA(Plasminogen Activator) ELISA Kit	PLGA
Human PLOD3(Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3) ELISA Kit	PLOD3
Human PLSCR2(Phospholipid Scramblase 2) ELISA Kit	PLSCR2
Human PLTP(Phospholipid Transfer Protein) ELISA Kit	PLTP
Human PLVAP(Plasmalemma Vesicle Associated Protein) ELISA Kit	PLVAP
Human pMAPT/pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit	pMAPT/pTAU
Human pNF-H (Phosphorylated Neurofilament H) ELISA Kit	pNF-H
Human PP(Placental Protein) ELISA Kit	PP
Human PP(Pepsin) ELISA Kit	PP
Human PP13(Placental Protein13) ELISA Kit	PP13
Human PP14(Placental Protein14) ELISA Kit	PP14
Human PPAR-α(Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit	PPAR-α
Human PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit	PPAR-γ
Human PPARγC1α(Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha) ELISA Kit	PPARγC1α
Human PPAR-δ(Peroxisome Proliferator Activated Receptor Delta) ELISA Kit	PPAR-δ
Human P-PKC(Phospho Protein Kinase C) ELISA Kit	P-PKC
Human PPOX(Protoporphyrinogen Oxidase) ELISA Kit	PPOX
Human PPP1R1A(Protein Phosphatase 1 Regulatory Subunit A) ELISA Kit	PPP1R1A
Human PRB1(Basic Salivary Proline Rich Protein 1) ELISA Kit	PRB1
Human PRB2(Basic Salivary Proline Rich Protein 2) ELISA Kit	PRB2
Human PRCP(Prolylcarboxypeptidase) ELISA Kit	PRCP
Human PRELP(Proline Arginine Rich End Leucine Rich Repeat Protein) ELISA Kit	PRELP
Human Preptin(Preptin) ELISA Kit	Preptin
Human PRKG1(Protein Kinase, cGMP Dependent Type I) ELISA Kit	PRKG1
Human Pro-ADM(Proadrenomedullin) ELISA Kit	Pro-ADM
Human ProGRP(Pro-Gastrin Releasing Peptide) ELISA Kit	ProGRP
Human pro-IL1β(pro interleukin 1 beta ) ELISA Kit	pro-IL1β
Human Pro-MMP-1(pro-Matrix Metalloproteinase 1) ELISA Kit	Pro-MMP-1
Human Pro-MMP-10(Pro-Matrix Metalloproteinase 10) ELISA Kit	Pro-MMP-10
Human Pro-MMP-13(Pro-Matrix Metalloproteinase 13) ELISA Kit	Pro-MMP-13
Human proPSA(precursor of Prostate Specific Antigen) ELISA Kit	proPSA
Human PROS(Protein S) ELISA Kit	PROS
Human PRR4(Proline Rich Protein 4, Lacrimal) ELISA Kit	PRR4
Human PRSS23(Protease, Serine 23) ELISA Kit	PRSS23
Human PRSS33(Protease, Serine 33) ELISA Kit	PRSS33
Human PS(Phosphatidylserine) ELISA Kit	PS
Human PSAP(Prosaposin) ELISA Kit	PSAP
Human PSPN(Persephin) ELISA Kit	PSPN
Human PTD(Pentosidine) ELISA Kit	PTD