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Anti-Cytokeratin7单克隆抗体 人源流式免疫印

迹 细胞角蛋白7抗体
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  • 询价
  • MAB3554
  • 2025年07月12日
  • Flow Cytometry;Western Blotting
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Flow Cytometry;Western Blotting

    • 宿主

      Mouse

    • 适应物种

      Human

    • 库存

      大量

    • 抗原来源

      0

    • 是否单克隆

      1

    • 规格

      100 μg

    Species Reactivity Key Applications Host Format Antibody Type
    H FC, WB, IC, IH(P) Mouse Purified Monoclonal Antibody
    Description:
    Anti-Cytokeratin 7, clone OV-TL 12/30
    Background Information:
    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry.
    Clone:
    OV-TL 12/30
    Specificity:
    Cytokeratin 7. Reacts with specific glandular-type epithelia, and most carcinomas derived there from.
    Key Applications:
    • Flow Cytometry
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry (Paraffin)
    Application Notes:
    Immunoblotting

    Immunohistochemistry on frozen tissue sections fixed with methanol. The antibody is also effective on paraffin embedded tissue sections that have been pretreated with 0.1% pronase/0.1% trypsin in PBS, 0.5% pepsin in 0.01N HCl or microwave pretreatment.

    Optimal working dilutions must be determined by the end user.
    Species Reactivity:
    Human
    Isotype:
    IgG1
    Immunogen:
    Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.
    Presentation:
    Liquid in PBS containing 0.09% sodium azide.
    Storage Conditions:
    Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    Gene Symbol:
    • KRT20
    • Cytokeratin-20
    • K20
    • CK20
    • CD20
    • MGC35423
    • Keratin-20
    • CK-20
    • KRT21
    Antibody Category:
    Cell Structure
    Antibody Sub-Category:
    Cytokeratins
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Purified
    Host:
    Mouse
    Concentration:
    1 mg/mL
    Antibody Type:
    Monoclonal Antibody

    Product Family Information

    Cytokeratin Antibodies

    Millipore’s Cytokeratin antibody demonstrates specificity against cytokeratin. See below for data and references for Millipore’s cytokeratin antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon.

    » More…


    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line. 详细描述见链接:http://www.millipore.com/catalogue/item/MAB3554

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