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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Calmodulin, Bovine Brain, High Purity
- 库存:
大量
- 供应商:
默瑞(上海)生物科技有限公司
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文献和实验Purification of Calmodulin from Bovine Brain
. Procedure (0-4℃): 1.About 1 kg of bovine brain (PelFreeze,frozen)is homogenized in 2 volumes of homogenizing buffer (50 mM MOPS,pH 7.0,1.0 mM 2-ME,1.0 mM EDTA)for 90 sec in a large Waring blender. 2.The homogenate is centrifuged at 10,000 x g for 1 hr
to reflect the tightness of the in vivo blood–brain barrier. The present protocol describes the setup of an in vitro coculture model based on primary cultures of endothelial cells from bovine brain microvessels and primary cultures of rat astrocytes
In the past two decades, thoroughly standardized mouse incubation time and brain lesion profile scoring assays have been developed to discriminate between prion strains. However, in these mouse infection experiments, large numbers of animals
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