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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
AbMole
- CAS号:
74431-23-5
- 规格:
50mg
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文献和实验Alkaline Southern Blotting Procedure
. Do not quench on ice, add the 95 C probe directly to 10 ml of 65 C hybe solution. Discard the prehybe solution and add the hybe solution. Hybridize at 65 C overnight. WASH STEP: High Stringency 20mM Sodium Phosphate buffer pH 7.2 Two changes at 65 C, 50
Bisulfite Modification for Analysis of DNA Methylation
an efficient procedure utilizing a newly developed high?concentration bisulfite solution. Protocols for this methodology are supplemented with discussions focused on chemical aspects of the bisulfite treatment. Curr. Protoc. Nucleic Acid Chem. 33:6.10.1?6.10.15
v468 Chapter 7 通过免疫组化来测定β-连环蛋白核定位
9.0. To prepare, dissolve 1.2 g Tris and 0.37 g EDTA in 1 L distilledwater and dissolve by stirring. The resulting solution shouldalready be at the required pH 9.0. 3. Steel pan for boiling slides (we use a high-sided asparagus pan). 4. Hot plate. 5. PBS
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