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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 应用范围:
Flow Cytometry;Immunoprecipitation
- 宿主:
Mouse
- 库存:
大量
- 适应物种:
Guinea Pig;Human
- 抗原来源:
0
- 是否单克隆:
1
- 规格:
100 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, H, not R | FC, IP, INHIB, not WB, not IH | Mouse | Purified | Monoclonal Antibody |
- Flow Cytometry
- Immunoprecipitation
- Inhibits Activity/Function
- Western Blotting
- Immunohistochemistry
FACS Analysis
Function blocking: 100% inhibition of alpha9beta1-mediated adhesion to the third fibronectin type III repeat in tenascin at <10 μg/mL.
Not effective for immunohistochemistry, immunocytochemistry or western blot.
Optimal working dilutions must be determined by end user.
- Guinea Pig
- Human
- ITGA9
- RLC
- ALPHA-RLC
- ITGA4L
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Murine L cells transfected with human alpha9. 详细描述见链接:http://www.millipore.com/catalogue/item/MAB2078Z
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- 作者
- 内容
- 询问日期
文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
; 100% ethanol 2 x 5 minutes, 90% Ethanol 5 minutes, 75% ethanol 5 minutes, H2 O 2 x 5 minutes and allow to dry. Store dry, dust-free. Typical Protocol (1) Immerse the slides in 1M sodium thiocyanate at 80C for 10 minutes. Rinse in H2 O and allow
free shotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet were resuspended in 265 μl TE
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
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