- ￥4500
- 义翘神州
- 40592-V08H40
- 北京
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
SARS-CoV-2 (2019-nCoV) Spike RBD(E406Q)-His Recombinant Protein
- 保质期：
12个月
- 供应商：
北京义翘神州科技股份有限公司
- 保存条件：
-20℃ to -80℃
- 规格：
100 µg
纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(E406Q)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(E406Q)) was expressed with a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Arg319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(E406Q)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.5 kDa.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验2F+DwTquooTcizAj6Sh40arj1e+iWJ2ispgsbT801bWi9OaaVDQ0u7N9Rf22Q7je3ypEkT/aXtAx/4gHh20RbetchuWXizve3YY/3L0LGHHqt8Cva+97zf/vEfvyYb4imWXpTxLz8m4rvgjodswc3X26Xnn2o3LbjZPv/Fv5GGuGa33/ZzO2n2HD17NAEWeUXwmQh44uy32U+uutL+69e+6jjedfsddvSxx3m8xNNFQ67wipqQKHyESZ35
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[ 见 15.3.2 ( 5 ) ] 。 17. 建议体外检测蛋白质性质之前去除 DHFR 片段，除非特定的实验需要，因为它会减小产量，使提纯步骤更复杂，也有可能改变蛋白质性质。建议将蛋白质重新构建到不含 DHFR 片段的质粒中。为了实现这一目标，我们选用了兼容的酶切位点，用 XhoI 和 Sal 分别克隆文库和 F 片段 DNA [ 1，2] 。 18. 过夜饱和培养物 1 :10 稀释至含 100 μg/ml 氨苄青霉素的 TB 培养基中，37°C 300 r/min 培养 90
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