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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
SARS-CoV-2 (2019-nCoV) Spike RBD(S359N)-His Recombinant Protein
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度> 85 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(S359N)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(S359N)) was expressed with a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Arg319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(S359N)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验日前,北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台,针对新型冠状病毒及其变异株,设计了编码新冠病毒刺突蛋白(Spike)受体结构域(RBD)的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗(circRNARBD
Cell Death Differ:中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制
上皮细胞线粒体凋亡,并导致肺水肿的发生。 图片来源:Cell death & differentiation 病毒相关蛋白与细胞蛋白相互作用影响细胞凋亡过程。作者首先分别将携带刺突蛋白(Spike protein, S 蛋白),核衣壳蛋白(Nucleocapsid, N 蛋白),膜蛋白(Membrane protein, M 蛋白)和包膜蛋白(Envelope protein,E 蛋白)真核表达质粒载体分别转染人肺上皮 H292 细胞和人血管内皮 EA.hy926 细胞,通过荧光分光光度法检测胞内活化
5位院士为代表,超12篇CNS大作,5月新冠研究汇总——全方位围攻新冠病毒,值得收藏
的鸡尾酒疗法也引起了大家的关注,不同的抗体可以识别结合域上不同的抗原表位,在未来的临床应用中能够避免免疫逃逸的理想单克隆抗体对。 5 月 8 日,军事医学科学院生物工程研究所陈薇院士团队和西湖大学周强团队强强联合,在预印本平台 bioRxiv 上线题为:A potent neutralizing human antibody reveals the N-terminal domain of the Spike protein of SARS-CoV-2 as a site
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