PTEN <i>SMART</i>pool® siRNA r

Transfection of siRNA oligos to peritoneal macrophages

  Use DeliverX Transfection from Panomics Modified protocol below Step 1:  Make complexes Note:  Do not scale up more than 3x Thaw siRNA and DeliverX reagent Dilute siRNA into buffer 1 10 uM siRNA  2.17 ul Buffer

siRNA protocols

sequences for the preparation of T7 polymerase transcribed siRNA are of the form G(N17)C. The first and last nucleotide constraints are required because T7 polymerase puts a G at the start of all transcripts. RNAs are synthesised from templates

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

of nucleic acids for phase determination in nucleic acid X‐ray crystallography. Int. J. Mol. Sci. 9:258‐271.    Soutourina, J., Wydau, S., Ambroise, Y., Boschiero, C., and Werner, M